Mouse Models of Innate Immunity | Taschenbuch

Cover: 9781493960361 | Mouse Models of Innate Immunity | Methods and Protocols | Allen | Buch

Rückseite: 9781493960361 | Mouse Models of Innate Immunity | Methods and Protocols | Allen | Buch

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Sprache: Englisch

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Beschreibung

The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.

Zusammenfassung

Includes detailed contributions from a diverse group of authors

Features both ex vivo and in vivo protocols that are lab-ready

Contains expert tips and key implementation advice to ensure successful results

Inhaltsverzeichnis

Conventional Murine Gene Targeting.- Production and Characterization of Humanized Rag2
-/-
c
-/-
Mice.- Isolation, Culture, and Functional Evaluation of Bone Marrow-Derived Macrophages.- Collecting Resident or Thioglycollate Elicited Peritoneal Macrophages.- Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils.- Assessment of Oxidative Metabolism.- Generation and Culture of Mouse Embryonic Fibroblasts.- Primary Ear Fibroblast Derivation from Mice.- Bone Marrow Derived Dendritic Cells.- Measuring T Cell Function in Innate Immune Models.- Bioassay for the Measurement of Type-I Interferon Activity.- Safe and Effective Mouse Foot-Pad Inoculation.- Delayed Type Hypersensitivity Models in Mice.- Mouse Model of
Staphylococcus aureus
Skin Infection.- Sepsis Induced by Cecal Ligation and Puncture.- Systemic Infection of Mice with
Listeria monocytogenes
to Characterize Host Immune Responses.- Mouse Model of Invasive Fungal Infection.- Endotoxin-Induced Uveitis in Rodents.- Bacteria-Mediated Acute Lung Inflammation.- Intranasal Influenza Infection of Mice and Methods to Evaluate Progression and Outcome.- Dextran Sodium Sulfate-Induced Murine Inflammatory Colitis Model.- Bacterial-Mediated Gastrointestinal Inflammation.-
Plasmodium berghei
ANKA (PbA) Infection of C57BL/6J Mice: A Model of Severe Malaria.- Characterization of HIV-1 Infection in the Humanized Rag2
-/-
c
-/-
Mouse Model.

Details

Erscheinungsjahr:	2016
Fachbereich:	Therapie
Genre:	Importe , Medizin
Rubrik:	Wissenschaften
Medium:	Taschenbuch
Inhalt:	xi 225 S. 7 s/w Illustr. 20 farbige Illustr. 225 p. 27 illus. 20 illus. in color.
ISBN-13:	9781493960361
ISBN-10:	1493960369
Sprache:	Englisch
Einband:	Kartoniert / Broschiert
Redaktion:	Allen, Irving C.
Herausgeber:	Irving C Allen
Auflage:	Softcover reprint of the original 1st edition 2013
Hersteller:	Humana
Verantwortliche Person für die EU:	Humana Press in Springer Science + Business Media, Heidelberger Platz 3, D-14197 Berlin, juergen.hartmann@springer.com
Maße:	254 x 178 x 14 mm
Von/Mit:	Irving C. Allen
Erscheinungsdatum:	27.08.2016
Gewicht:	0,46 kg