Bioanalytics

Analytical Methods and Concepts in Biochemistry and Molecular Biology

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Beschreibung

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids.<br> <br> The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird's eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists.<br> <br> The following techniques are covered in detail:<br> <br> - Purification and determination of proteins<br> - Measuring enzymatic activity<br> - Microcalorimetry<br> - Immunoassays, affinity chromatography and other immunological methods<br> - Cross-linking, cleavage, and chemical modification of proteins<br> - Light microscopy, electron microscopy and atomic force microscopy<br> - Chromatographic and electrophoretic techniques<br> - Protein sequence and composition analysis<br> - Mass spectrometry methods<br> - Measuring protein-protein interactions <br> - Biosensors<br> - NMR and EPR of biomolecules<br> - Electron microscopy and X-ray structure analysis<br> - Carbohydrate and lipid analysis<br> - Analysis of posttranslational modifications<br> - Isolation and determination of nucleic acids<br> - DNA hybridization techniques<br> - Polymerase chain reaction techniques<br> - DNA sequence and epigenetic modification analysis<br> - Analysis of protein-nucleic

Artikeldetails

EAN

9783527339198

Sprache

Englisch

Einband / Art

Buch

Maße

282 x 220 x 55 mm

Erscheinungsjahr

2018

Verlag / Hersteller

Wiley-VCH GmbH